M [CRO-ORG W ISMS OF M Al'l.i-; S KT 



341 



organisms into the tap-hole. Sterile metal spouts and buckets were 

 used and the covers employed were fastened to prevent blowing 



in the wind. The sap was gathered daily, and after each col- 

 lection the buckets were thoroughly scalded in order to main- 

 tain practical sterility. The plates poured from sap obtained on 

 March 26 and April 6 appear in table 3. 



TABLE 3. ORGANISMS PEE CC. 1 N SAT OBTAINED l\ STERILIZED CONTAIN! BS 



BACTERIAL. CONTENT OF SAP OBTAINED UNDER SEPTIC CONDITIONS 

 AS COMPARED WITH THAT OBTAINED UNDER ASEPTIC CONDITIONS 



Trees which were running sour sap were retapped about 4 

 inches to one side of the old tap-hole. Sterile spouts and buckets 

 were hung at the fresh wounds, while the sour spouts and buckets 

 were allowed to remain undisturbed except that the sap was 

 emptied from the old bucket at the time the clean one was hung 

 at the fresh tap-hole. The sap was allowed to run from each 

 spout for a few hours, after which samples were collected and 

 plated. The results are tabulated below in table 4. 



TABLE 4. ORGANISMS PER CC. IX SAP FROM FRESH AND FROM SOUR TAPHOL1 S 



