Micro-organisms of Maple Sap 



351 



Since it was deemed important to conduct the inoculation 

 experiments with sap from the first runs only, about ioo trees 

 were tapped for experimental purposes as early as it seemed 

 probable that any sap could be obtained. 



DETAILED DISCUSSION OP SAMPLES 



SERIES I : SIRUPS I TO 6 



The sirups of this series were made from the first run of 

 the season which occurred on March 24, 1909. It remained in 

 the woods over night and was brought to the field laboraton 

 the following- morning- and divided into six portions. Four <>i 

 these were inoculated and two retained as controls. They were 

 held for three days, the controls being kept at a temperature of 

 from o° to +io° C. The incubation temperature of the inocu- 

 lated samples is indicated in the temperature graph (fig. 2). 



Fig. 2. Graph of incubation temperatures for series 1 ; saps 1 to G In- 

 clusive. The arrow heads on next to the bottom line from left to right indi 

 cute respectively the time of inoculation, the time of evaporation of the first 

 sample and the time of evaporation of the last sample. 



