8f)4 Bulletin 167 



36. Number 36 was inoculated with red yeast CII. The 

 sap clouded promptly but only a very few colonies of the organ- 

 ism were recovered. The plates showed a count of 7,312,000 in 

 which the fluorescent group predominated. The sirup contained 

 96.16% sucrose and 0.61% invert sugar. The color was 9, 

 flavor 2 1 , and score 725; depreciation from control, color 5, 

 flavor o 1 , and score 150. 



37. Number 37 was inoculated with red yeast CLXXV. 

 The organism w-as not recovered. The plates gave a count of 

 8,825,000 per cc. with pronounced fluorescence. The sirup con- 

 tained 95.88% sucrose and 0.64% invert sugar. The color was 

 11, flavor 2 1 , and score 675; depreciation from control, color 7, 

 flavor o 1 , and score 200. 



38. Number 38 was the control placed in the incubator af- 

 ter heating to 8o° C. The plates showed a count of 162,500 

 colonies per cc. of which very few developed fluorescence. The 

 sirup contained 94.80% sucrose and 0.48% invert sugar. The 

 color was 5, flavor 2 1 , and score 825 ; depreciation from cold 

 control, color 1, flavor o 1 , and score 50. 



39. Number 39 was inoculated with gray yeast C. Green 

 souring developed and the organism was not recovered. The 

 plates showed a count of 9,750,000 colonies per cc. with pro- 

 nounced fluorescence. The sirup contained 96.18% sucrose and 

 0.26% invert sugar. The color was 7, flavor 2, and score 800; 

 depreciation from control, color 3, flavor o, and score 75. 



40. Number 40 was a control placed in the incubator with- 

 out heating. Plates showed a count of 5,850,000 organisms with 

 the fluorescent group in predominance. The sirup contained 

 95.05% sucrose and 0.64%^ invert sugar. The color was 9, flavor 

 2, and score 750; depreciation from cold control, color 5, flavor 

 o, and score 125. 



The results of this series confirmed the suspicion entertained 

 from the inoculation trials of the previous season's work, that the 

 yeasts did not develop readily in maple sap, but that the bac- 

 teria normally present gained the ascendency. The changes in 



