Micro-organisms oi J Maple Sap 369 



sucrose and 3.96^ invert sugar. The color was 6, flavor 3, and 

 score 750; depreciation from control, color 4, flavor _\ and 



score 225. 



54. Number 54 was inoculated with pink yeast ( WW I. 

 A deep milky appearance developed to a pronounced degree and 

 an unpleasant yeasty odor was noted. The organism was re- 

 covered, the plates showing- a count of 1,300,000 organisms per 

 cc. The reaction was 8.5% N/100 acid. The sirup contained 

 94.11% sucrose, and 2.71 % invert sugar. The color was 7, 

 flavor 2 2 , and score 750; depreciation from control, color 5, 

 flavor i J . and score 225. 



55. Number 55 was inoculated with a non-fluorescent or- 

 ganism CVIII. A milky type of souring developed in which a 

 brownish color was evident in strong light, and a peculiar bac- 

 terial odor was noted. The organism was recovered in prac- 

 tically pure culture. The plates showed a total count of 7,230,- 

 000 organisms per cc. The reaction was 6% N/100 acid. The 

 sirup contained 94.76% sucrose and 2.38% invert sngar. The 

 color was 5, flavor 2, and score 850; depreciation from control. 

 color 3, flavor 1, and score 125. 



56. Number 56 was the incubator control heated to boil- 

 ing and placed in the incubator with the other samples without 

 inoculation. It clouded slightly during the incubation period. 

 The plates poured revealed a bacterial count of 320,000 per cc. 

 The reaction was 3.1% N/100 acid. The sirup contained 96.029? 

 sucrose and 1.35% invert sugar. The color was 5, flavor 2, and 

 score 850; depreciation from control, color 3, flavor 1, and score 



125- 



57. Number 57 was the control not heated and retained 



outside the incubator at low temperature during the incubation 

 period. At the time of evaporation it had a temperature of 

 io° C. The plates showed a development of 37,000 bacteria 

 per cc. The reaction was 2.5% N/100 acid. The sirup con- 

 tained 96.47% sucrose and o.6o r r invert sugar. The color was 

 2, flavor 1, and score 975; depreciation from control (number 



