372 Bulletin 167 



showed a development of 97,500 colonies per cc., most of which 

 were characteristic of the introduced organism. The reaction 

 was 10% N/100 acid. The sirup contained 91.64% sucrose 

 and 5.86% invert sugar. The color was 8, flavor 3, and score 

 700; depreciation from control; color 5, flavor 2, and score 250. 



65. Number 65 was a control retained in the incubator. 

 The sap clouded early and appeared like the inoculated samples. 

 The plates showed a development of 1,950,000 colonies of vari- 

 ous species. The reaction was 9% N/100 acid. The sirup 

 contained 91.89% sucrose and 3.52% invert sugar. The color 

 was 6, flavor 4, and score 625 ; depreciation from control (num- 

 ber 58), color 3, flavor 3, and score 325. 



influence; of the container upon the quality of the sirup 



Under the above heading will be discussed the sirups made 

 from the saps previously referred to in connection with the 

 discussion of the influence of the container upon the bacterial 

 content of the sap (page 343). It will be remembered that 

 six trees were employed which had been tapped with special 

 rare and upon which tin buckets were hung. For the purpose 

 of a part of this experiment, they were replaced by wooden 

 "ins. The tin buckets were kept free from the accumula- 

 tion of micro-organisms by frequent washings and scaldings. 



66. Number 66 was made from sap collected -March 21 

 and 22, in clean tin buckets. The composite sample from the 

 6 trees contained 140 organisms per cc, most of them of a single 

 type previously mentioned as characteristic of tree 3 (Plate VI >. 

 The reaction was 2% N/100 acid. The sirup contained 96.62% 

 sucrose and 0.41^' invert sugar. The color was 3, flavor I, and 

 score 950. 



67. Number 67 was made from sap obtained from the same 

 trees as was number 66 but it flowed two days later on March 23 

 and 24. The tin buckets were replaced by wooden buckets 

 formerly employed in the sugar place where the work was done. 

 They had been soaked out in the usual way and thoroughly 



