M [CR0-0RGA1N [SMS OP M \ri i; S VP 





si:k i i:s 9: sirups j.\ to 8] 



The sap for this series was gathered in pails and broughl 

 to the laboratory about to p. m., March 24. It was carefully mixed 

 and divided into 18-quart portions, all but one of which were 

 heated to boiling. The following morning the samples which 

 had been heated were placed in the incubator and inoculated. 

 with the exception of one which was reserved as an incubator 

 control. The air temperature maintained during the incu- 

 bation period may be seen by referring to the accompanying 

 graph (figure eo). 



Fig. 10. Graph of incubation temperatures for series 9 ; saps 74 to si in 

 elusive. The arrow heads on next to the bottom line from lefl to ri^'lii indl 

 cate respectively the time of inoculation, the time of evaporation of the firsl 

 sample and the time of evaporation of the last sample. 



74. Number 74 was the control which was not heated. It 

 was evaporated on the morning of March 25. The sap contained 

 750 organisms per cc. The reaction was 3% N/100 acid. Un- 

 fortunately the sirup was slightly burned in the pan so that its 

 color and flavor were seriously impaired. For this reason the 

 sirups of this series have been referred to number 68 as a con- 

 trol. Sirup 74 contained 96.36% sucrose and 0.70' J invert sugar. 

 The color was 9, flavor 4, and score 550; depreciation from con- 

 trol (number 68), color 5, flavor 3, and score 375. 



75. Number 75 was inoculated with fluorescent organism 

 CLXXIX. The plates showed a development of 10.400,000 

 colonies per cc. divided between those characteristic of the 

 fluorescent group and those of another organism of the subtil is 

 type. The reaction was 10% N/100 acid. The sirup contained 

 84.74% sucrose and 10.77% invert sugar. The color was 9, flavor 

 3. and score 675 ; depreciation from control, color 5, flavor 2. 

 and score 250. 



