Micro-organisms oj? Maple Sap ; '7'.» 



jo but not quite as dark as that of number 85. The flavor was 

 3 1 , and score 325. 



SIRUP FROM LAST RUN SAP IN I9IO 



The plan pursued in the following experiments was exactl) 



the same as that described for similar experiments the previous 

 year (page 357). The first trees were retapped April 3. Il 

 was the intention to continue the experiments upon different 

 trees until the flow of sap was so reduced that it was impossible 

 to secure enough to make even a small sample of sirup. Unfor- 

 tunately for the purpose of the work the weather became warm 

 and night freezes ceased altogether, so that only a single experi- 

 ment of this character was possihle. 



87. Number 87 was secured from the fresh tap-hole of a 

 tree which had yielded sour sap for a considerable number of 

 days and upon which the leaf buds were already opened. The 

 sap contained 5 organisms per cc. The reaction was 2.5'/ N/100 

 acid. The sirup contained 97.27% sucrose and 0.26% invert 

 sugar. The color was 4, flavor 5, and score 525; depreciation 

 from first run control, color o, flavor 3, score 250. 



88. Number 88 was made from the same tree as number 87 

 but from the sap flowing from the old tap-hole. The plates gave 

 a count of 73,125,000 organisms per cc. The reaction was 4% 

 N/100 acid. The sirup contained 94.56% sucrose and r.79% in- 

 vert sugar. The color was 11, flavor 6, and score 250; deprecia- 

 tion from control (number 87), color 7, flavor 1, and score 275. 

 .Depreciation from first run control, color 7, flavor 4, score 625. 



The results afforded by these two samples arc interesting 

 and significant, for it will be ohserved that the flavor of the last 

 inn material in 1910 was very seriously impaired, even when bac 

 teria were excluded, the typical buddy flavor being present in an 

 unmistakable degree, while the color remained light. Manifestly 

 the poor flavor of No. 87 can not be attributed to the influence 

 of the bacteria present for they were practically absent, but 5 per 

 cc. being found. Its cause must be sought elsewhere ; and naturally 



