398 Bulletin 167 



Discussion of Related Sirups in Groups 



A Mirvey of the preceding- table shows that the sirups natu- 

 rally fall into different groups according to the character of the 

 sap used or of the treatment accorded to it. For convenient 

 study and discussion the tables have been re-arranged so as to 

 bring similar samples together in groups. 



It appeared in the discussion of individual samples that the 

 inoculation failed in certain instances. Such samples have been 

 placed by themselves and are grouped together without regard to 

 the character of the organisms with which inoculation was at- 

 tempted. In one series a spore-bearing organism became at least 

 equally as important as the bacteria which were artificially intro- 

 duced, and this series has been treated as a unit in the re-arrange- 

 ment. The samples inoculated with yeasts and molds showed a 

 tendency to mixed infection in which the fluorescent organism^ 

 naturally present played a more or less important part. With the 

 other statistical data in the following tables, the average color, 

 flavor and score for each group, as well as the average deprecia- 

 tion of color, flavor and score, is recorded at the foot in the 

 proper columns. 



Twenty-two sirups were made from sap successfully inocu- 

 lated with one or another strain of fluorescent bacteria. The 

 average color was 9, flavor 2.0. and score 605 ; the average depre- 

 ciation from control, color 4.8, flavor 1.6, and score 242. 



Four samples were successfully inoculated with non-fluo- 

 rescent bacteria. The average color was 5.5, flavor 2.5, and, 

 score 800: the average depreciation from control, color 0.5, flavor 

 JA. and score 100. 



Seven samples were influenced by the action of a spore- 

 bearing organism of the subtilis type which appeared sponta- 

 neously. Four of these were inoculated with fluorescent organ- 

 isms, one with a non-fluorescent bacillus similar to the intruder, 

 and one with a pink yeast which was not recovered, while the 

 other was intended for an incubator control. It is evident that 

 the quality of these sirups must lie attributed to the combined 

 action of the two groups of organisms. The average color was 



