Micro-organisms of Maple Sap 390 



8.4, flavor 3.6, and score 618; the average depreciation from 



control, color 4.4, flavor 2.6, and score 307. 



Three sirups were made from sap successfully inoculated 

 with Bacillus aceris, a stringy sap organism, of which two strains 

 were employed. The average color was 6.7, flavor 3.7 and score 



650; the average depreciation from control, color 2.7, flavor _\ 

 and score 242. 



Six samples were inoculated with pink cocci belonging t« > the 

 Micrococcus roscus type. The average color was 5.7. flavor 2.6, 

 and score 775; the average depreciation from control, color 2.8, 

 flavor 1 .f>, and score 171 . 



( )nly one sample was successfully inoculated with pink yeast. 



Thirteen samples were inoculated with red yeast with at least 

 partial success. The fluorescent bacteria developed in associa- 

 tion with the introduced organisms in a considerable number of 

 these samples, so that the quality of sirup secured must he 

 assigned to the combined activities of the two species. The 

 average color was 8, flavor 2.8, and score 703; the average de- 

 preciation from control, color 4.1, flavor 1.7, and score 215. 



Eight samples were inoculated with gray yeast with partial 

 or complete success. Here again the fluorescent bacteria some- 

 times developed in association with the yeasts and the results 

 must be regarded as the product of the activities of both clasps 

 of organisms. The average color was 7.3, flavor 3.2, and score 

 078; the average depreciation from control, color 3.8, flavor 1.7. 

 and score 234. 



Four samples were successfully inoculated witli green mold. 

 In two instances Penicillium was employed and in the others, 

 Eurotium. Fluorescent bacteria played a part as associated or- 

 ganisms in certain of these samples. The average color was 9, 

 flavor 3.5, and score 600; average depreciation from control. 

 color 4.8, flavor 2.0, and score 281. 



Four sirups were made from saps inoculated with a mixture 

 of organisms. The composite infection was undertaken to de- 

 termine the influence of yeasts and molds upon the development 

 of fluorescent bacteria. The results were not entirely satisfactory 



