450 Bulletin 167 



they all appear to exert a marked inverting action on sucrose. 

 The total and insoluble ashes tog-ether with malic acid value 

 are above standard in all cases. 



Group 14. Tin z's. wooden buckets. The sap from 6 trees 

 was used in this phase of the work. All tap-holes, spouts and 

 utensils were made strictly clean and the procedures were iden- 

 tical in both cases. The sap was concentrated as soon as col- 

 lected. That obtained in the tin buckets ran 2 days earlier than 

 that gathered in the wooden buckets, but, as a check, in order 

 to be certain that the two days' intermission exercised no in- 

 fluence on the results, bacterial counts were made on sap ob- 

 tained in tin buckets from the same trees and spouts after the 

 close of the experiment. It was practically free from bacteria 

 of any kind; (see page 343). The grade of sirup produced 

 from the tin buckets (Xo. 66) was of the highest quality, rank- 

 ing 1 in flavor and 3 in color, and scoring 950. That secured 

 from the wooden buckets (No. 67), in marked contrast to that 

 made when tin buckets were employed, ranked only 4 in flavor, 9 

 in color and scored but a total of 550 points out of a possible 

 975. Furthermore its invert sugar content was high, there be- 

 ing seventeen times as much present in the sirup secured in the 

 wooden buckets as was found in that made when tin buckets were 

 used. This is doubtless to be attributed to the organisms al- 

 ready existing in the wooden buckets since, although thoroughly 

 cleansed, they were not new and were painted on the out side- 

 only. 



Both sirups were light in weight containing respectively 

 37.78 and 38.33 perccnts of water. 



The sucrose is considerably higher in Xo. 00 than in Xo. 

 67, owing to its low invert sugar content. 



The standards for ash and malic acid value are fully met 

 by both samples. 



