4:78 Bulletin 167 



approximately 50 C. Optimum temperature 25 C. Maximum 

 temperature 37 C. Minimum temperature not determined. 

 Growth slow at io° C. 



12. Desiccation. — Growth occurred after drying for nine 

 days in some cases, but not after drying for 20 days. 



13. Insolation. — Exposure on ice to direct sunlight at noon 

 in August ion fifteen minutes killed 46.7^ of the organisms. 



14. Acids produced. — Not identified. 



15. Alkalies produced. — Ammonia. 



16. Alcohols produced. — Ethyl alcohol. 



17. Ferments produced — The organism digests gelatin 

 slowly: yet notwithstanding all attempts to demonstrate pro- 

 teolytic ferments by the milk serum method gave negative re- 

 sults. Potato starch was evidently acted upon feebly, alcohol 

 being produced, but diastatic ferments could not be demonstrated. 

 Invertase formed in very small quantities. 



18. Effect of germicides. — Formalin and phenol were tested 

 in varying: amounts in bouillon. Phenol retarded growth in 

 dilutions of 1-1000 and killed the bacillus in dilutions of 1-500. 

 Formalin retarded growth in dilutions of 1-2750 and inhibited 

 growth in dilutions of 1-2200. 



19. Number. — According to the numerical classification of 

 the Descriptive Chart of the Society of American Bacteriologists 

 the organism is Bacillus 221. 11 13022. 



Detailed Descriptk in 

 occurrence 



At various times during the progress of the study of the 

 bacteria of maple sap. stringy or ropy specimens of the material 

 were encountered. These were of two types. The more common 

 form was usually observed only after the close of the commercial 

 season and was associated with the presence of filamentous fungi, 

 yeasts, and various bacteria growing together. This sap always 

 presented a more or less lumpy appearance when poured from the 

 bucket. The other type of stringy sap was of a more uniform 



