Micro-organisms of M aim.k Sap P.* I 



Litmus milk was prepared by adding- 0.10% of azolitmin 1" 

 fresh centrifuged milk and filtering through several thicknesses 

 m!" filter paper. Acid production became apparent within 24 hours 

 at 20, 25 and 30 C. followed by gas production, coagulation, 

 and extrusion of the whey, as reported under milk. Gradual hut 

 complete reduction of the litmus was observed beginning at the 

 top of the tube about the sixth day and becoming complete about 

 the sixteenth day. No further change was observed in tubes 

 held under observation for 3 months. 



Starch jelly was prepared according to the directions given 

 by Smith. The nutrient material employed was the same author's 

 modification of Uschinsky solution. Good growth occurred upon 

 this within 24 hours. A broad band of raised growth, higher at 

 the border than at the center, with a papillate surface, an echinu- 

 late edge and a faint opalescence, resulted. Slight local lique- 

 faction of the medium was noted on the sixth day accompanied by 

 a change from opalescence to opaqueness with gas production in 

 the medium. On the tenth day and at intervals thereafter for six 

 weeks, tubes were examined by washing with distilled water. 

 Portions of the starch jelly had been rendered soluble so that 

 pockets were left in the surface. The wash- water and dissolved 

 material was passed through filter paper and tested for sugar 

 with Fehling's solution, with negative results. A portion of the 

 filtrate was distilled and the distillate tested with sodium hydroxid 

 and iodine as described under alcohol production. Iodoform was 

 produced, indicating the presence of alcohol, aldehyde, or ketone. 

 From the fact that Fehling's solution was not reduced the prob- 

 ability is strongly in favor of the supposition that the body was 

 an alcohol. 



Cohn's solution proved an unfavorable medium for the culti- 

 vation of this organism. Cultures held under observation for 21 

 days failed to show growth. 



Uschinsky solution. — Cultures developed a copious growth 

 appearing within 24 hours, accompanied by a pellicle formation 

 which after about 3 days sank and deposited as a membranous 



