4Q2 Bulletin 167 



sediment. The medium was slightly viscid at the end of 2 weeks. 



Fermi's solution inoculated with organisms developed a slight 

 growth within 24 hours, followed by the formation of a thin 

 pellicle which sank and formed a thin membranous sediment. 

 The growth was transient, the tubes becoming almost clear in 

 3 days. 



Silicate jelly containing Fermi's solution showed a light 

 growth, becoming apparent the second or third day as a thin, 

 pearly white, surface development. The medium and growth 

 thereafter remained unchanged. 



Sodium chlorid in bouillon. — Transfers were made to bouil- 

 lon ( + 10 Fuller's scale) containing 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 

 II, and 12%, respectively, of chemically pure sodium chlorid. 

 Growth promptly appeared in the four weaker solutions, but was 

 very slightly restricted in 5% sodium chlorid. It invariably ap- 

 peared upon the 6% and 7% solutions within 4 days and upon 

 the 8% and 9% ones in from 3 to 7 days. No growth occurred 

 in 10%, 11%, or 12% sodium chlorid bouillon within 21 days. 

 Transfers from these tubes into normal bouillon gave no growth. 

 In no case was development entirely inhibited by less than 10% 

 of sodium chlorid, but cultures in solutions containing from 5% 

 to 9% were not characteristic. Microscopic examination showed 

 organisms to be grouped together in chains in the 5% solution. 

 No signs of motility were observed in this or stronger concentra- 

 tions. In the broths containing in excess of 5% the growth was 

 restricted to clustered colonies gathered on the sides of the tubes. 

 In solutions up to 4% growth was apparently normal, the group- 

 ing and motility being characteristic. 



Grozvth in bouillon over chloroform was unrestricted and 

 characteristic. 



Maple sap sterilised by heating at 99 to ioo° C. on three 

 consecutive days and inoculated with a 2 mm. loop of the organ- 

 ism, developed a deep milky appearance within 24 hours. Growth 

 was accompanied by the formation of mucilaginous gum on the 

 walls of the flask in contact with the culture fluid. This layer 



