Micro-organisms of Man.k Sap t95 



out within 15 hours after inoculation, while at 25 and 30 ^;is 

 production was well under way. The growth in lactose and 

 sucrose was similar to that in dextrose but less pronounced. 

 Maltose was especially favorable for the development of the or- 

 ganism and abundant gas production was obtained upon this 

 sugar. Glycerin broth appeared a good medium for growth in 

 the open arm, but only restricted growth occurred in the closed 

 arm, and no gas was produced. Mannit gave good growth in 

 both the open and closed arms accompanied by gas production. 

 The tables showing acid production follow. 



