Micro-organisms of Maple Sap 7,11 



i. A solution containing- 2^ of phenol, 2% of sucrose, and 

 ._•' , of dextrose. 



2. A solution containing 2'/< of phenol, 2% of sucrose, and 

 .2% of dextrose, together with an equal volume of sterile bouillon. 



3. A solution containing- 2% of phenol, 2% of sucrose, .2' - 

 of dextrose and an equal volume of a io day old broth culture of 

 the organism. 



4. A solution containing 2% of phenol, and 2 r /< of sucrost 



5. A solution containing 2% of phenol, and _>' , of sucrose, 

 together with an equal volume of sterile bouillon. 



6. A solution containing 2'.', of phenol. _>', of sucrose. 

 together with an equal volume of a 10 day old broth culture of the 

 organism. 



7. A solution containing 2% of phenol. 



8. A solution containing 2% of phenol, together with an 

 equal volume of sterile bouillon. 



9. A solution containing 2% phenol, together with an equal 

 volume of a 10 day old broth culture of the organism. 



10. A solution containing sterile bouillon. 



11. A solution containing- a 10 day old broth culture of the 

 organism. 



Three 10 cc. portions A. B, and C of each solution were taken. 

 To the solutions A, 1 cc. of a strong solution of lead acetate wa^ 

 added. They were filtered, the excess of lead acetate precipitated 

 with potassium oxalate crystals, care being taken to avoid an 

 excess of potassium oxalate. The lead oxalate was filtered off 

 and the filtrate reserved for treatment with Fehling's solution. 

 Portions B were treated with 1 cc. of copper sulphate solution 

 used in connection with the Fehling's solution. Portions C were 

 given no preliminary treatment before using the Fehling test. 

 The precipitation was carried out in Erlenmeyer flasks. By 

 means of a pipette 10 cc. of the copper solution was placed in the 

 flask, then 10 cc. of the tartrate, care being taken to avoid gather- 

 ing undissolved precipitate on the walls. The two were mixed 

 and heated t<» boiling. A 10 cc. portion of the solution to he 



