516 Bulletin 167 



NAME 



A careful comparison of the cultural and biochemical char- 

 acters of this organism with those of bacteria previously de- 

 scribed would seem to justify its recognition as a new species. 

 The name Bacillus aceris (new species) is therefore suggested 

 for it. 



B. BRIEF DESCRIPTION OF THE PINK COCCI OF 



MAPLE SAP 1 



Pink colonies were frequently found upon the plates poured 

 from sour sap, particularly late in the season. Some of these 

 were produced by yeasts or yeast-like organisms while others were 

 composed of micrococci. Four strains were selected for further 

 study from the cultures of pink micrococci isolated. These were 

 CXXVI, CIV, XL'IX. and CVI. A brief description of the or- 

 ganisms is given here for the purpose of record. All appear to 

 belong to the type of Micrococcus roscus. They resemble each 

 other closely and will be described as though identical, any varia- 

 tions in behavior from the general type being noted as they occur. 



Morphology 



Vegetative cells. — The organisms art.' coccoid in form, occur- 

 ring singly, in twos, fours, or irregular packets. Division is in 

 two planes. The average diameter as determined on agar hang- 

 ing 1 block cultures was 1.2 microns. The smallest organisms ot>- 

 served measured .8 micron and the largest 2. microns. Cultures 

 in nutrient broth had a tendency to be somewhat smaller and to 

 occur singly or in twos. 



Spores. — Evidences of spore formation were absent. 



Flagella.— -The organisms were non-motile and no indica- 

 tions of flaij'ella were observed. 



'The matter appearing on pages 516-521 is briefed from a thesis 

 entitled "The Pink Micro-Organisms of Maple Sap." presented by N. R. 

 Smith, B. S., of the class of 1911, in partial fulfillment of the require- 

 ments for the baccalaureate degree in the College of Agriculture of the 

 University of Vermont. 





