518 I'.PI.I.KTIN 167 



Potato. — Organisms failed to develop visible growth upon 

 cooked potato slants. 



Gelatin stab. — Growth was slow, moderate, at first beaded 

 almig the line of puncture, becoming filiform above. Slight lique- 

 faction began on the twenty-fourth day and continued slowly, 

 becoming' stratiform in six weeks. The liquefied gelatin became 

 pinkish, contained a compact pink sediment and was distinctly 

 viscid. 



Gelatin plate.— Colonies appeared the third day, and attained 

 a diameter of 2 mm. in 10 days. They were convex with round 

 entire edges. The pigment upon gelatin was similar to that upon 

 agar except that it was usually of a darker hue. 



Broth. — First evidences of growth consisted of a slight, com- 

 pact, pinkish sediment appearing the second or third day, while 

 clouding appeared in one w r eek. The sediment was viscid on 

 agitation. 



Milk. — The first evidence of growth consisted of a depositr m 

 of pink sediment, becoming apparent within the first 10 days. In 

 3 weeks the medium was colored light pink while a watery ap- 

 pearance developed the fourth week. This appeared to be as-- > 

 ciated with feeble digestive action, although complete peptoniza- 

 tion did not occur. Coagulation did not take place. 



Litmus milk. — The organisms studied developed considerable 

 variation upon this medium. CXXVI showed acid formation 

 after 14 days, with coagulation 2 or 3 days later, promptly fol- 

 lowed by the reduction of the litmus in the lower part of the tube. 

 Partial digestion of the curd occurred. The first evidences of 

 peptonization appeared during the third week and progressed 

 slowly until about the ninth week, when about half of the total 

 curd had become dissolved. CIV exhibited no change in the 

 medium until after 6 weeks, when it turned alkaline and curdled. 

 Digestion did not occur. XLIX became alkaline the third week 

 and developed a soft curd 3 or 4 days later. Litmus reduction 

 occurred after 4 weeks. Partial liquefaction of the coagulum was 

 observed in about half of the cultures vi this strain, but did not 



