Micro-organisms os M\m Sap 519 



occur in the remainder. CVI curdled milk after 5 weeks with 

 reduction of litmus. The color was restored with au alkaline re- 

 action in the upper half of the tube after 8 weeks. No digestion 

 of casein was observed. 



The variations upon litmus milk were so great that the work 

 was repeated several times and the purity of the cultures carefully 

 verified, but without change in the character of the reaction. 



Cohn's solution. — A slow growth was noted which was char- 

 acterized by the development of sediment which usually was 

 granular and flaky, but sometimes slightly viscid. 



Uschinsky solution. — The growth was slow, at first moderate 

 in amount, becoming abundant after 5 weeks, with copious sedi- 

 ment. A portion of the culture immediately above the sediment 

 exhibited clouding. 



Growth in bouillon over chloroform. — There was no growth 

 111 bouillon tubes to which 2 or 3 drops of chloroform were added. 



Physical and Biochemical Features 



Acid production. — Nutrient broths to which were added 2' , 

 of various sugars and 5^? of glycerin respectively were tested for 

 gas formation and acid production in fermentation tubes. In no 

 case was there sufficient gas production to become apparent with 

 the Smith tubes. Ten cc. portions were titrated in the usual way 

 at the end of I, 2, 4, 10, and 28 days. The reaction of duplicate 

 checks was subtracted from the average reaction of inoculated 



luplicates to determine the average acid or alkali production. 



The results expressed in percenl normal are shown in the follow- 

 ing- table. 



f 1 * 



