528 Bulletin 167 



stains were applied on material from many media and, except for 

 polar bodies, were without result. 



5. Capsules. — Broth cultures of the three strains, XXXIII, 

 CXY, and CXLA , were commonly stringy. Capsules were demon- 

 strated on these strains, both by Welch's method and by Richard 

 Muir's contrast stain. Attempts to demonstrate capsulation on 

 some of the other strains were unsuccessful. 



6. Stains. — All strains, except CXV, were Gram negative, 

 though decolorization was not always complete, granulations in 

 the rods and polar bodies being common. Strain CXV retained 

 Gram's stain after 4 minutes in absolute alcohol. 



. Iqueous anilin stains. — Cells of all strains were easily stained, 

 but gave up the dye readily on washing - . The rods often showed 

 granulation or a bi-polar effect, certain portions seeming to have 

 a greater affinity for the stain than others. 



II. Cultural Features 



1. Agar stroke. — Growth abundant, filiform to echinulate, 

 spreading below; raised, smooth, glistening and somewhat viscid 

 or slimy. Medium more or less green fluorescent with ah strain-. 

 Strain CXV showed at most only a doubtful fluorescence. 



2. Potato. — Growth moderate with all strains. A rather 

 narrow, smooth, filiform, light brown streak, becoming thick and 

 spreading later. Medium sometimes greened but not invariably. 

 Strain CXV often showed a dull rhizoid growth. 



3. Agar stab. — Best development at top of puncture but 

 considerable sub-surface growth later. Filiform, becoming vil- 

 lous. Varying degrees of green fluorescence. Long crystals 

 were common in old cultures: they occurred under the surface 

 growth and about the puncture and were never detected in control 

 tubes. Similar crystals are illustrated by Smith (27:66). Beau- 

 tiful green fluorescence, varying somewhat in intensity with the 

 various strain-. Strain CXY was doubtfully fluorescent. 



4. Gelatin. — Nutrient broth, stiffened with 10. 12, and 15' > 

 gelatin, was used. Several complete sets of the strains were 



