Micro-organisms of M\ru, Sap 531 



appearing in from 15 to 23 days. Those strains showing no 

 growth were transferred to broth after twenty (lavs. The trans 

 fers developed normally becoming fluorescent in two days. 



11. Uschinsky solution. — The medium was prepared as 

 follows : 



Distilled water, 1000. cc. 



Glycerin (Merck's Blue Label), 10. " 



Sodium chlorid, 5. gr. 



Calcium chlorid, 0.1 " 



Magnesium sulphate, 0.4 " 



Di-potassium phosphate, 2.2 " 



Sodium asparaginate, 4. " 



All strains except CXV developed well <>n this medium, 

 showing strong fluorescence, and a viscid pellicle and sediment. 

 Transfers made from tubes of CXV on Uschinsky solution de 

 veloped well in broth. 



12. Nitrogen requirements. — For determining the nitrogen 

 requirements, a modified Uschinsky solution prepared as follows 

 was used as a basal medium. 



Distilled water, 1000. cc. 



Glycerin (Merck's Blue Label), 35. " 



Sodium chlorid, G. gr. 



Calcium chlorid, <».l " 



Magnesium sulphate, 0.35 " 



Di-potassium phosphate, 2. 



The mixture was warmed to secure complete solution and 

 divided into three portions, one of which was reserved as a con- 

 trol while the others were treated respectively with 0.5', of c. p. 

 urea and 1% of asparagin. 



No growth, or only a slight trace, occurred in the contn<l 

 medium. The feeble trace of transient growth in a portion of 

 the controls was attributed to the traces of nitrogen known to 

 be present in the glycerin. 



Upon the urea medium all showed good growth, except 

 strain CXV which failed to develop. Transfers of this strain 

 from the urea medium to broth developed promptly. 



