582 Bulletin 167 



Eight strains developed with fluorescence upon the asparagin 

 medium. These were as follows: CXXIX, CXXXIII, CXLVIII, 

 CXLIX, CLIX, CLXXYII. 5 and LVI. Transfers to nutrient 

 broth from the asparagin cultures of those strains which failed 

 to grow developed promptly. 



III. Physical and Biochemical Features 



1. The action of the organisms upon dextrose, lactose, 

 sucrose and glycerin. — The carbohydrate and glycerin media 

 were made by adding 2% of the sugars and 5% of glycerin 

 respectivelv to bouillon which was sugar free as shown by the 

 B. coli test. Cultures were observed for acid and gas produc- 

 tion and for growth in the closed arm of the Smith tube. 



Cultures and controls were analysed in duplicate for acid 

 production after 1, 2, 4, 10, and 20 days incubation. Five cc. 

 of the material were pipetted into 45 cc. of distilled water in 

 Erlenmeyer flasks; j/ 2 cc. of 1% phenolphthalein solution was 

 added and the mixture boiled 2 minutes and titrated hot with 

 N/20 sodium hydroxid. Boiling often increased the amount 

 of free acid, probably through the volatilization of ammonia 

 formed simultaneously with the acid. 



Control titrations were made upon bouillon cultures. Some 

 acid was detected which is thought to have resulted from the 

 decomposition of proteids, since muscle sugar was absent. 

 The conclusions as to acid production which are indicated in 

 the group number, pages 548 and 550, were drawn after com- 

 paring the results on the carbohydrate media with those on in- 

 oculated bouillon. 



No gas was produced from the sugars or glycerin. Growth 

 in the closed arms of the fermentation tubes was limited and oc- 

 curred only in old cultures. 



The detailed discussion of the characters on various car- 

 bohydrates follows : 



Dextrose. — Acid production without gas evolution was char- 

 acteristic. Two '< dextrose litmus agar developed an acid 



