548 Bulletin 167 



greater than before." Jordan believes that the fluorescent prop- 

 erty is purely incidental. "When the medium contains cer- 

 tain compounds the metabolic activities of the organisms adjust 

 themselves to these conditions and it is of no physiological 

 significance that one of the products of this activity happens to 

 be fluorescent." 



13. Diastasic action on potato starch. A thin starch 

 paste containing 2% thymol was added to equal volumes of 

 ten day old broth cultures, the tubes placed in the thermostat 

 for 8 hours, filtered and the filtrate tested with Fehling's solu- 

 tion. Sugar was detected in none of the cultures, indicating 

 that diastase had not been formed. 



14. Group numbers. — The characteristics of the several 

 strains detailed above correspond as follows. 



12 strains to the number, Pseudomonas 221.2332132 



12 " " " " " 221.2332133 



7 " " " " " 221.2322132 



4 " " " " " 221.2322133 



2 " " " " " 221.2333133 



1 " " " " " 221.2323132 



2 " " " " " 221.2222132 



1 " " " ' " 221.2232133 



1 " " " " Bacillus 221.2222?32 



However since there is no sharp line between acid pn >- 

 duction and no acid production from the sugars and glycerin. 

 the only specific difference brought out by the group number is 

 in regard to nitrate reduction. If gelatin cultures had been hell 

 only six weeks to determine liquefaction, the group number oi 

 strains CXL. CXLI, CXLII. CXUII. CXLAT, XVI, and L, 

 would have been 222.2332133 and of strains LI and IJV 222.- 

 2 333 x 33- For the group number corresponding to the respec- 

 tive strains, see page 550. 



15. Separation of the 42 strains into groups. — The 42 

 strains naturally separate themselves into 6 groups with re- 

 spect to the following properties : the production of hydrogen 

 sulphid, the reduction of nitrates to nitrites, growth upon as- 

 paragin Uschinskv solution, Cohn's nutrient solution, gelatin. 



