Micro-organisms of Maple Sap 555 



slants by means of Loerfler's method (with anilin gentian violet i 

 or by Lowit's modification of the same. The arrangement of 

 the flagella of the several strains was as follows. 



Strain CXII. — A polar tuft of 3 flagella at one end of a 

 chain of two cells was typical. Occasionally tufts of 1 to 6 

 flagella occurred at both ends of double cells. 



Strain CXI'.— This strain had peritrichiate flagella. Tufts 

 were common at each end of chains. Lateral flagella were com- 

 mon on single cells (c. f. Plate X, figure 2). 



Strain CXL. — The flagella typically occurred in a polar tuft 

 at one end of the double cells, frequently at both ends. 



Strain CXL]'. — Tufts of flagella were demonstrated on one 

 end of double cells, and occasionally on both ends. Single or- 

 ganisms usually had one polar flagellum. 



Strain CXLVIII. — Double cells commonly had 1 or 3 flagella 

 at one end ; occasionally 1 at each end. Single cells with 2 polar 

 flagella were not uncommon. 



Strain XXX]' I. — The double cells had 1, 3, or 6 polar 

 flagella, most commonly a tuft of 3 ; occasionally the flagella 

 occurred at both ends of the double cells. 



Strain LI. — Most commonly 2 flagella occurred at one end 

 of the chains of two cells and 3 at the other, or 1 at one end 

 and 1 to 6 at the other. Double cells with I to 6 flagella at 

 one end were common. 



4. Capsules. — Only the fluorescent sap bacteria were ex- 

 amined for capsules. Such an envelope was demonstrated upon 

 strains CXV and CXLV and upon strain XXXIII as previously 

 noted (page 528). A suggestion of capsulation was noted on 

 flagella preparations of strain CXLVIII, but the results with the 

 contrast capsule stain were negative. Cultures of strain XXXVI 

 (in 2% sucrose peptone solution were very stringy. 



Strain CXV. — Capsules were easily demonstrated upon 

 preparations from 10 day broth cultures by either Welch's 

 method or by Richard Muir's contrast stain (22:106). Fre- 



