Micro-organisms Or' Maple Sap -''''I 



while filiform line which gradually became echinate to villous. 

 Liquefaction proceeded from crateriform to stratiform and was 

 completed in about one month. Fluorescence was rarely seen in 

 -j-io gelatin but appeared in alkaline gelatin (compare with Ps. 

 mesenterial and Ps. fluoresceins pages ^' ? n, 560). An abundant 

 white flak}- precipitate settled out as liquefaction proceeded. 

 Growth in the puncture was transient, the line of inoculation re- 

 maining about the same. 



Strain CXV. — Growth appeared in a few hours, the puncture 

 being beaded, liquefaction becoming evident in [6 hours. The 

 colony sank in a saucer-like depression, the liquefaction soon 

 becoming stratiform, proceeding quite rapidly at first but grad- 

 ually slowing down when nearly all the 7 cc. of medium had been 

 liquefied. The line of puncture quite frequently showed con- 

 siderable liquefaction. The fluid was slightly turbid but devel- 

 oped neither yellow nor green color. A thin surface membrane 

 developed and a moderate amount of white precipitate was thrown 

 down. Liquefaction of the 7 cc. of medium was completed in 

 about a month's time. 



Strain CXLV. — This organism at the beginning of the studies 

 was one of the most rapid liquefiers, commencing liquefaction in 

 24 hours and completing it in 27 days : but in the course of 18 

 months' cultivation it has gradually lost its ability to liquefy 

 gelatin reacting -f-10 Fuller's scale. Otherwise it does not differ 

 essentially from strain CXII. In the studies with alkaline gela- 

 tin media with original reactions of — 4.7 and — 12.4 Fuller's scale, 

 this organism showed crateriform liquefaction in 26 days in the 

 — 12.4 medium and none in the — 4.7 medium. At this time the 

 — 12.4 medium had a reaction of -f-2.7 Fuller's* scale. It is thus 

 evident that this organism retained the power to liquefy alkaline 

 or very slightly acid gelatin, after it had practically lost it- 

 ability to liquefy the ordinary gelatin. Fluorescence occurred 

 only in the alkaline gelatin. 



Strain CXLVIII. — This organism resembled l's. fluorescens 

 very closely on gelatin stab, but in old cultures it did not show 



