Micro-organisms of M ai'i.k Sap 563 



to beaded, becoming villous later. Liquefaction constantly began 



after about 4 mouths and proceeded slowly, the fluid being thick 

 and slimy. At this time the stab was a faint white filiform line, 

 development in this portion of the culture having ceased. Ac 

 cording to Migula's classification Ps. tenuis is a non-liquefier. 



( 21 \(J\o). 



Strain CXL. — Cultures of this organism were like those of 

 Ps. tenuis. Liquefaction constantly began after about 5 months' 

 incubation. At this time the stab was either filiform and beaded. 

 or filiform, beaded, and widely villous. As in the cultures of Ps. 

 tenuis the liquid was quite thick and slimy. The purity of the 

 culture and its identity with the original strain was proved. It 

 is worthy of note that this organism, when held at 25 s C. pep- 

 tonized milk only after three months' incubation. 



6. Nutrient broth. — Upon this medium slight difference- 

 were observed among the different strains. Moderate clouding 

 appeared in 18 to 24 hours in all except CXV, which was rather 

 tardy in showing growth in most liquid media. The medium 

 rapidly became more or less turbid, forming a scum or pellicle 

 which at intervals settled to the bottom of the tube as a white 

 flaky to viscid precipitate. A rather tough membranous pellicle 

 was produced by stain CXLY. More or less blue-green fluo- 

 rescence was apparent in from 3 to 10 days, but was never strong 

 in Ps. longa, Ps. tenuis. Ps. putrida or XXXVI, except in alkaline 

 broth. Strain CXV produced only a trace of fluorescence. The 

 other strains usually showed beautiful green fluorescence, but 

 it was not constantly present. The blue-green rapidly became 

 yellow-green and in old cultures the medium cleared and showed 

 an amber yellow color. Cultures of CXV and CXI A often 

 became quite stringy. 



Strain CXI'. — A slight to moderate clouding appeared in 

 from 2 to 5 days, gradually increasing in intensity. Later the 

 cultures developed a white surface membrane, the substratum 

 becoming quite clear. An abundant white precipitate was thrown 

 down. Old cultures became amber colored. 



