Micro-organisms of M \ru Sap 565 



There were _> types among the i,^ strains according to their 

 action on milk. One type produced considerable acid and i 

 agulated and digested the medium quite rapidly, a more or less 

 greenish color being present. The coaguhun was of a more or 

 less jelly-like consistency. The other type showed clearing with- 

 out coagulation of the milk, the action being delayed and taking 

 place very slowly. With certain strains digestion was not evi- 

 dent until about the third month. To the first group belong the 

 following strains: Ps. fluorescens, CXII, CXV, CXLVIII, and 

 XXXVI; and to the second group belong: Ps. alba, Ps. longa, 

 Ps. mesenterica, Ps. tenuis. Ps. putrida, CXL, CXLV, and LI. 



Strains Ps. fluorescein, CXII, and CXLVIII of the first 

 type, behaved similarly on milk. A more or less jelly-like co- 

 agulation appeared in from 2 to 10 days coincident with acid 

 production. The coagulum formed was rather slimy with the 

 first organism named but with the others was quite firm. Diges- 

 tion with more or less greenish discoloration of the peptonized 

 portion was apparent in 4 days, becoming complete in from 18 to 

 30 days, the cultures at this time being relatively clear and closely 

 resembling broth cultures of similar age. A deep green color 

 appeared at the surface in these old cultures and upon agitation 

 the whole medium became olive green. The coloration was more 

 pronounced in Ps. fluorescens than in the other two. Luxwolda 

 (18) states that B. fluorescens liquefaciens first coagulates milk 

 with a lab ferment and then peptonizes it. 



Strain XXXVI differed from the three preceding organisms 

 in that only a trace of green color was present. Coagulation with 

 this strain was always of jelly-like consistency. 



Strain CXV showed clearing without coagulation until about 

 the eighteenth day when a firm coagulum appeared coincident 

 with acid production. Digestion continued and was completed in 

 from 30 to 104 days. The medium never appeared green but was 

 more or less straw colored throughout. 



With the eight strains of the second type the medium re- 

 mained practically unchanged in appearance from ro to 85 days, 



