

Micro-organisms of Maple Sap 567 



The second group, non-liquefiers, (and very tardy liqui 

 and digesters), comprises the following strains: /'.v. alba, Ps. 

 longa, Ps. putrida, Ps. tenuis. CXL, U and Ps. mesenterial. 

 Alkalinity was apparent the second day. except with Ps. putrida; 

 it first manifested this reaction on the tenth day. Alkalinity grad 

 ually increased at first in cultures of this group, and after some 

 time acidity developed simultaneously with digestion. After 

 several months, when digestion was nearly complete, cultures ol 

 all strains except CXL and Ps. putrida were colored purple 

 throughout, without perceptible clearing. Cultures of CXLwi 

 pink throughout, but otherwise resembled Ps. putrida. 



The action of all [3 strains on litmus milk was essentially 

 the same; first, alkali production, then acid production accom- 

 panied by digestion. 



9. Gelatin colonics. — The colony of strain CXL VII] is de- 

 scribed as the type of the rapidly liquefying group. 



Macroscopically ; at first punctiform to round, yellowish, 

 raised, smooth, glistening, and entire, soon liquefying and sink- 

 ing in saucer-like depressions, the fluid showing slight green 

 fluorescence. Microscopically; round, entire, convex, brownish, 

 center dark and surrounded by a thinner zone (liquid). In- 

 ternally granular to grumose with spiny processes about the edge. 

 The filaments were variously oriented, being more loosely inter- 

 twined at the vd^v where they extended out into the liquid por- 

 tion. The colonies soon disintegrated, becoming floccose or 

 grumose masses in the liquefied gelatin. (See I'latc XII-XR I. 



Ps. fluorescens and strains CXI I and CXI A' could not he 

 distinguished specifically from CXIA 111. 



Strain CXV. — This strain closely resembles the type. Radiate 

 filaments were frequently present, often being almost perfectly 

 symmetrical, the ends of the filaments forming the bordering 

 fringe of the colonies. Liquefaction was very rapid. 



Strain XXXVI. — At first round, entire, yellowish brown, 

 finely granular discs, sometimes with an irregular cracked appear- 

 ance. Some were undulately zoned and the surface irregularly 



