572 Bulletin 167 



This was filtered and divided into two portions, one of which 

 was used full strength and the other diluted with an equal volume 

 of water. They were autoclaved, and inoculated at the same time 

 from the same stock cultures. 



The results attained are summarized in the following- table : 



TABLE 63. USCHINSKY SOLUTION 



Normal solution Diluted solution 



Strain Fluo- Fluo- 



Growtli Time rescence Time Growth Time rescence Time 



Modified Uschinsky solution was also prepared as follows : 



Distilled water 1000. cc. 



Glycerin (Merck's Blue Label) 30. gr. 



Sodium chlorid 6. " 



Calcium chlorid 0.1 " 



Magnesium sulphate (tested purity) 0.4 " 



Di-potassium phosphate 2. " 



Ammonium lactate 6. " 



Sodium asparaginate 4. " 



This was filtered and divided into two portions, one of which 



was used full strength, w r hile the other was diluted with an equal 



\<>lume of water. Yet another solution was prepared leaving 



out the ammonium lactate. This was likewise divided into two 



portions, one being used at full concentration, the other at half 



concentration. The four media were inoculated at the same time 



from the same cultures. 



