Micro-organisms of Maple; Sap 



.. , .1 



chemicals: asparagin, sodium asparaginate, urea, potassium 

 nitrate, calcium nitrate, ammonium lactate, ammonium phosphate, 



ammonium tartrate. The ten media were inoculated at the same 

 time from the same cultures. 



TABLE G6. NITROGEN REQUIREMENTS 



Strain 



Ps. alba. 



Ps. fluorescens, 



Ps. longa, 



Ps. mrsent erica. 



Ps. tenuis 



Ps. putrida, 



CXII, 



CXV, 



CXL, 



CXLV, 



CXLVIII, 



XXXVI, 



LI, 



Ps. alba. 



Ps. fluorescens, 



Ps. longa, 



Ps. mesenterica, 



Ps. tenuis. 



Ps. putrida, 



CXII, 



CXV, 



CXL, 



CXLV, 



CXLVIII, 



XXXVI, 



LI, 



Aspar- 

 agin 



G. F. 



+ 

 + 

 + 



+ 

 + 

 + 



Sodium 

 aspara- 

 ginate 



G. F. 



+ 

 + 

 + 

 + 



+ 



Urea 

 G. F. 



+ 



Potassium 

 Peptone nitrate 



+ + 



+ + + + 



+ 

 + 

 + 

 + 

 + 



+ 

 + 



+ 

 + 



+ + 

 - + 



+ + 



G. 



+ 

 + 

 + 

 + 

 + 

 + 

 + 

 + 

 + 

 + 

 + 

 + 

 + 



F. G. F. 



+ 



+ 



+ + + 



+ 

 + 

 + 

 + 

 + 



Calcium 

 nitrate 



G. F. 



Ammo- 

 nium 

 lactate 



G. 



+ 

 + 

 + 

 + 

 + 



+ 



F. 



+ 

 + 



+ 



Ammo- 

 nium 

 phosphate 



C. F. 



Ammo- 

 nium 

 tart rate 



G. F. 



+ + 



+ 

 + 



+ 

 + 



+ 

 + 

 + 



+ 

 + 

 + 

 + 

 + 



Note.— G., growth; F., fluorescence; +, observed; — , not observed. 



Observations upon these media were continued for over a 

 month. Peptone was the only source of nitrogen used by all 

 strains. Except with strain CXV, good growth was apparent in 

 24 hours; the latter showed good growth in 3 days. All strains, 

 except Ps. putrida and CXV, were able to utilize asparagin and 



