Micro-organisms oi? Maple Sap 58] 



TABLE 70. lM'ol. PRODUCTION IN DUNHAM'S PEPTONE 



Strain. 2 days G days 11 days 26 d 



Ps. alba, ± + 



Ps. fluoresce us. ± 



Ps. longa, — — ± 



Ps. mesenterica, — — 



Ps. tenuis, — — • 



Ps. putrida, — — — — 



CXII, 



CXV, ± ± 



CXL, + + 



CXLV, 



CXLVIII, t 



XXXVI, 

 LI, — • — — 



5. Toleration of acids and alkalies. — Three liters of nutrient 

 broth were prepared in the usual way and divided into 13 por- 

 tions. These separate lots were adjusted, by titrating hot against 

 phenolphthalein with N/20 sodium hydroxid and N/20 hydro- 

 chloric acid, to the following reactions: — 37-5; — 30.0; — 23.8: 

 — 13.5;— 11. o;— 7.3:0.0; +8.8; +14.6; +25.0; +34.0 Fuller's 

 scale. 



Since alkaline media rapidly change in reaction owing to 

 carbon dioxid absorption, control tubes of the several . media 

 were subjected to the same conditions as the cultures and their 

 reactions determined after 1 and 3 days. Table 71 indicates the 

 results secured upon these alkali and acid media after 24 hours' 

 incubation. The original reactions of the several media and, also, 

 the reactions after 24 hours are given at the top of the table. 

 Table 72 indicates the reactions of the media and the growth of 

 the organisms after a 3 days' incubation. 



