596 Bulletin 167 



III. Physical and Biochemical Features 



1. Action upon carbohydrates in peptone solution. — Neither 

 acid nor gas were formed in 1% peptone solutions containing 

 2% of either dextrose, lactose, sucrose or glycerin. 



2. Ammojiia production. — Moderate ammonia production; 

 18.6 cc. N/10 hydrochloric acid equivalent produced in 100 cc. of 

 broth in 10 days. 



3. Nitrates in nitrate broth. — Not reduced. Nitrates and 

 ammonia present ; ammonia probably from proteid decomposition. 



4. Indol production. — Doubtful in 11 day Dunham's pep- 

 tone cultures after boiling ; absent in nutrient broth. 



5. Toleration of acids and alkalies. — Slight. 



6. Optimum reaction for growth. + 15 Fuller's scale. 



7. Vitality on culture media. — Long. 



8. Temperature relations. — Thermal death point; between 

 50.2 and 50.5 C. Optimum temperature approximately 25 C. ; 

 maximum, 37 ° C ; minimum, 5-7 C. 



9. Resistance to desiccation. — Continued vitality after 3 

 days drying on cover slips ; 4 days fatal. 



10. Insolation. — Sensitive ; 80 to 100% killed after 10 

 minutes' exposure. 



11. Acids produced. — Abundant hydrogen sulphid produc- 

 tion. 



12. Alkalies. — Moderate ammonia production. 



13. Relation to oxygen. — Strict aerobe. 



14. Group number. — Bacillus 21 1.3332 ?33. 



BRIEF CHARACTERIZATION OE THE 12 STRAINS OE Ps. fluOreSCeUS 



For the convenience of any one who may have occasion to 

 review these results the brief characterization items of the six 

 strains of fluorescent bacteria isolated from maple sap together 

 with those of strains alba, fiuorcscens, longa, mesenterica, tenuis, 

 and puirida are summarized in tabular form on pages 598 anil 

 599- 



