13 



black growth of Macrosporium was confined to a portion of the 

 spot, the remainder being covered with a lighter green growth, which 

 proved to be Cladosporium. Chains of apical spores characteristic 

 of Alternaria were also found. In all three cases the mycelium grew 

 in a felty mass near the surface, agreeing in every respect with that 

 described by Galloway and others previously quoted. The average 

 size of the outdoor spot was perhaps larger than that in the green- 

 house. Cultures were made of these fungi and from them green fruit 

 was inoculated, both on the vine and under a bell-jar, but all inocu- 

 lations failed to infect. 



BACTERIAL ROT. 



In the late spring of 1904, another crop of greenhouse tomatoes- 

 was set out for the same work. The spot was present in abundance 

 from the first and lasted through August, viz., until the plants were 

 on the decline, as before. Out of more than a hundred diseased 

 tomatoes which were cut up and placed in a moist chamber, only ten 

 showed signs of Fusarium growth, and none of these were covered 

 with the vigorous felty mycelium which always developed in 1902. 

 In only one case was a pure growth obtained, the Fusarium being 

 slow to appear and accompanied by various molds, as well as bac- 

 teria. Again a pure culture of the Fusarium was obtained and 

 twelve inoculations made, all of which produced characteristic spots. 

 The constant and early appearance of the bacteria led to a trial of 

 these for inoculation. 



CULTURAL CHARACTERISTICS. 



The various Petri dish cultures taken from the rotting tomatoes 

 were inoculated by piercing the tissue just beneath the skin with a 

 flamed needle, after cutting through the spot with a sterilized knife. 

 Out of two hundred or more cultures made during the season one 

 characteristic organism was isolated. The deep and surface 

 colonies showed different forms as described below : 



Description of the colonies in agar plate cultures : 

 Deep colonies — Round — fusiform, often elliptical, entire, porcelaneous 

 changing to butyrous, refraction strong, homogeneous, 

 coarsely granular, size .17 — .05 millimeters after 

 twenty-four hours. 



