*4 



■Surface colonies — Round, convex, refraction weak, grumose, entire, 

 butyrous, with nucleus. 



The growth of the organism in various media is as follows : 



Agar-Agar — Comparatively slight growth, white. 



Gelatin — Liquification, crateriform changing to stratiform in three 



days. 

 Litmus gelatin — A band of yellow through the center of medium after 



four days; after nine days, white with bluish ring at 



top. 

 Litmus lactose agar — Heavy cream colored growth ; medium blue 



after three days. 

 Milk — No coagulation. 

 Litmus milk — A slightly alkaline reaction. 

 Potato — In three days a thick, firm, tawny growth ; slight growth 



after. 

 Slices of green tomato — Dense growth of a tawny color, darkening 



with age. 



From pure cultures of the above organism, sixty-seven inoculations 

 were made during August, and many which were unrecorded before 

 that time. Out of the sixty-seven, fifty inoculations produced a well 

 developed and characteristic spot within a few days. (See Fig. 6.) 

 Of those made in July a larger percentage was successful, and there 

 was also more rot by natural infection. From the spots artificially 

 produced, the organism was isolated repeatedly. 



The position and general appearance of the spot during this 

 season were identical with that of 1902. The boundaries were clear 

 cut, and the surface, until late in the season, free from fungi. In 

 several cases, however, late in August, the fungi occurred as on the 

 outdoor crop, and again unsuccessful attempts were made to inocu- 

 late with these. There were slight differences to be noticed in the 

 color, consistency and markings of the rot in the two crops under 

 consideration. In 1904, the average spot was darker, there were 

 practically no concentric rings or veins visible on the surface, and 

 the tissue was often tender rather than leathery. 



The successful inoculations with two organisms led to the idea of 

 trying others not taken from the spot. As work on soil bacteria was 

 going on in the laboratory at this time, three of these cultures were 



