J 5 



used for inoculation. Six inoculations were made from each culture. 

 The inoculations made with two of these were unsuccessful, but 

 those in which the third was used produced a rot which was not to 

 be distinguished from that by natural infection. After this, several 

 clean punctures were made in tomatoes on the vine by means of a 

 flamed needle. No rot set in for a week or more, after which a very 

 characteristic spot developed in two of the tomatoes which were 

 close to the ground. It cannot be definitely inferred in either of 

 these experiments that the rot was caused by an organism other than 

 the one studied, as no attempt was made to isolate an organism 

 either in the case of the soil bacteria or the clean punctures. 



Fig. 6. Photograph of three tomatoes : One dis- 

 eased by natural infection, one by inoculation with 

 bacteria and one uninfected. 



DISCUSSION OF RESULTS. 



From the investigations recorded, we draw the following con- 

 clusions : 



First, That Fusarium so/an/, Mart., is the initial and in all proba- 

 bility the only active parasitic fungus connected with the fruit rot of 

 tomato. The reasons for this may be summed up as follows : 



i. Both Galloway 1 and Whitehead 2 determined a Fusarium in 

 connection with the disease. In both cases it is described as pene- 



