BY DR. KLEIN. 113 



the water are added gradual!}-. "Tnrnlmir* /.'///<-." Ten 



grains of protosulphatc of iron are dissolved in an ounrr ,f 

 glycerin diluted with a little water. Thirty-two grains of ferro- 

 cyanide of potassium are dissolved in the same quantity. The 

 iron is then added to the red prussiate with constant agitation. 

 Beale modifies this formula by substituting five grains of sul- 

 phate of iron and ten of the red prussiate for the quantities 

 above stated, and adding to the mixture an ounce of water 

 and a drachm of alcohol.] 



Carmine. A mass which is fluid at ordinary temperature 

 is prepared, according to Beale, as follows : Take five grains 

 of carmine, half an ounce of glycerin containing eight or ten 

 drops of acetic acid, one ounce of pure glycerin, two drachms 

 of alcohol and six drachms of water. The carmine is first 

 mixed with a little water containing about five drops of ammo- 

 nia. Half an ounce of pure glycerin having been added to 

 this liquid, it is shaken in a flask, and then gradually poured 

 into the acidulated glycerin, with constant agitation. If the 

 mixture is not distinctly acid, a trace of acetic acid is added to 

 the remaining half ounce of glycerin, which with the alcohol 

 and water is then gradually added to the rest. It is necessary 

 to prepare this mixture each time that it is used. The alcohol 

 ma} r be omitted altogether without detriment. Carmine is 

 usually employed in solution of gelatin. The following liquids 

 are to be recommended : 



Gerlach's Carmine Mass. Sixty-nine grains of carmine are 

 dissolved in se vent}' grains of water witli eight drops of liquor 

 ammoniae. The solution, having been exposed to the air for 

 several days, is mixed with a solution of one and a half drachm 

 of gelatin in one and three-quarter drachm of water. A few 

 drops of acetic acid are added to the warm mixture. Dr. Car- 

 ter's Carmine Mass. Take sixty grains of carmine, 120 

 grains of liquor ammoniae, eighty-six minims of glaciai acetic 

 acid, two ounces of solution of gelatin, containing one part 

 in six. one and a half ounce of water. The carmine is dis- 

 solved in the ammonia and water and filtered. The filtrate is 

 added to one and a half ounce of solution of gelatin. The 

 other half ounce is mixed with the acetic acid, and added gut- 

 tatim to the rest, with constant agitation. 



I found this mass answer extremely well with the following 

 modification: Four grammes of carmine having been sus- 

 pended in a few drops of water, eight cubic centimetres of 

 liquor ammonia? and forty-eight cubic centimetres of water are 

 added. As soon as the carmine is dissolved, the liquid is fil- 

 tered a process which requires several hours. A gelatin so- 

 lution, containing one part in eight of gelatin, is next prepared 

 and filtered through fine calico. The carmine solution is added 

 gradually to two ounces of the filtrate, which is kept warm 

 8 



