BY DR. KLEIN. 173 



hair pencil) and then excised and stained in silver solution, 

 may be placed, in the manner above described, in an inflamed 

 conjunctiva. If the preparation is taken out after twenty-four 

 hours, and studied immediately on the warm stage, we are able 

 to satisfy ourselves that, in those parts which exhibit the char- 

 acteristic silver staining, young cells are actually found in the 

 canaliculi, and pass along them. 



Of mammalia, } r oung rabbits answer best for studies of the 

 cornea. Inflammation is excited by the same methods. The 

 results are also similar. In a cornea excised twenty-four hours 

 after thorough cauterization, and stained with gold, parts are 

 found in the strips which are obtained by the method previously 

 described, in which the canaliculi assume the character of 

 channels of even width, which, as well as the cell cavities, are 

 lined with chains of small cells, arranged in linear series, so 

 as to resemble endothelial elements. From these appearances, 

 we are justified in concluding that both the bodies and the pro- 

 cesses of the cornea corpuscles have split into 3 r oung elements, 

 changing, at the same time, their form. 



Inflammation of the Tongue of the Frog. In the 

 tongue, cell division can be studied both in the corpuscles 

 peculiar to the organ and in migratory cells. For this purpose, 

 the tongue is prepared as for the study of the circulation. The 

 mucous membrane covering the large lymphatic sac of the under 

 surface is snipped off with curved scissors. The observation 

 is necessarily tedious, often lasting for forty-eight hours. It 

 is therefore desirable to replace the tongue in the mouth for a 

 time after each examination. 



Inflammatory Changes in the Tadpole's Tail. The 

 inflammatory changes which take place in branched cells may 

 be studied in those of the tadpole's tail. In a curarized tad- 

 pole, the required degree of irritation can be produced either 

 by simply pencilling the surface, or by allowing a drop of am- 

 monia to fall on it from a capillary pipette, or, finally, by piercing 

 it witli a needle. The research must be continued often for 

 many hours. The results are similar to those observed in the 

 cornea, and may be studied either in the fresh state or in gold 

 preparations. 



