ANILIN COLOURS GIVING INDIRECT NUCLEAR STAINS. 59 



CHAPTER VIII. 



ANILIN* COLOURS GIVING INDIRECT NUCLEAR STAINS 

 (FLEMMING'S METHOD), AND DIRECT NUCLEAR STAINS. 



A. Indirect Nuclear Stains. 



95. VEEY few anilins give a precise nuclear stain by the 

 direct method ( 92). Two of them methyl green and Bis- 

 marck brown are pre-eminently nuclear stains. Many of 

 the others for instance, safranin, gentian, and especially 

 dahlia may be made to give a nuclear stain with fresh tis- 

 sues by combining them with acetic acid ; but in ninety-nine 

 cases out of a hundred are not so suitable for this kind of 

 work as the two colours first named, which practically form a 

 class apart. 



Again, very few anilins give a pure plasmatic stain (one 

 leaving nuclei unaffected). The majority give a diffuse stain 

 which in some few cases becomes, by the application of the 

 decoloration or indirect method (92), the most precise and 

 splendid stain as yet obtainable by any means. 



The indirect staining method, or Flemming's method, and 

 the direct nuclear stains will form the subject of the present 

 chapter, and the remaining anilins will be treated of in the 

 next chapter. 



The following list shows the colours treated of in the two 

 chapters. 



In Chap. VIII. A. ( Colours giving indirect nuclear stains, 

 FLEMMING'S method.) Safranin, gentian violet, dahlia, Victoria 

 blue, anilin green, Magdala red (naphthalin red, rose de 

 naphthaline), mauvein, rouge fluorescent, solid green, fuchsin 

 (anilin red, rubin, rosein, magenta, solferino, corallin), orange, 

 Bismarck brown, methyl violet, tropaeolin 000, No. 2, (orange 

 II, chysaurein, j3 naphthalorange), rocellin (echtroth, or- 



* The word " anilin " is here used in the popular sense, to include all 

 coal-tar colours. 



