60 ANILIN COLOURS GIVING INDIRECT NUCLEAR STAINS. 



seillin No. 3, rubidin, La Rawvarienne), benzoazurin. B. 

 (Colours giving direct nuclear stains.) Methyl green (me- 

 thanilin green, vert lumiere, lichtgriin, griinpulver, vert en 

 cristaux), Bismarck brown (Manchester brown, phenylen 

 brown, vesuvin, La Phenicienne), methyl violet (methyl anilin, 

 anilin violet, Paris violet, inchiostro di Leonardi), methyl 

 violet 6 B, fuchsin. 



In Chap. IX. A. Methylen blue. B. (Plasma and 

 ground stains.) Bleu lumiere (Parma blue, toluidin blue, 

 lichtblau), bleu de Lyon (bleu de nuit, Griinstichblau), in- 

 dulin (nigrosin, bengalin, anilin blue-black, blackley blue, 

 artificial indigo), quinolein blue (cyanin, chinolinblau), ben- 

 zoazurin,, anilin blue, violet B., anilin black (blue-black, ni- 

 granilin,noir de Colin), Saiirefuchsin (fuchsin S., acid fuchsin), 

 Congo red (Congoroth), benzopurpurin, delta purpurin, Bieb- 

 richer scharlach (Biebrich scarlet), eosin (primerose, ery- 

 throsin, pyrosin B., rose B. a 1'eau), Bengal rose, picric acid, 

 metanil yellow (metanilgelb), saiiregelb (echtgelb), tropaeolin 

 0., crocem, gold orange, iodine green (Hofmann's griin), 

 thiophen green, anilin green, malachite green. 



General Directions for the Indirect or " Flemming " staining 



Method.* 



96. Staining 1 . Sections only can be stained by this method. 



The solutions employed are made with alcohol, water, or 

 anilin, according to the solubility of the colour. There seems 

 to be no special object in making them with alcohol if water 

 will suffice, the great object being to get as strong a solution 

 as possible. The sections must be very thoroughly stained in 

 the solution. As a general rule they cannot be left too long 

 in the staining fluid. With the powerful solutions obtained 

 with anilin a few minutes or half an hour will frequently 

 suffice, but to be on the safe side it is frequently well to 

 leave the sections twelve to twenty-four hours in the fluid. 

 Up to a certain point the more the tissues are stained the 

 better do they resist the washing-out process, which is an 



* Historically the principle of this method is due to HEEMANN and 

 BOETTCHEB; but it is universally known by the name of Flemming, to 

 whom is due the credit of having greatly improved the method in its prac- 

 tical details. 



