156 COMBINATION STAINS. 



the preparations have the aspect of injections. The connective 

 elements are stained of a nearly pure blue, so that it is easy 

 to distinguish them from the nervous elements. 



Applicable to all kinds of tissues, but especially to sections 

 of nerve-centres. 



Recent authors recommend, instead of anilin blue, bleu de 

 Lyon, dissolved in 70 per cent, alcohol acidulated with acetic 

 acid (MAURICE and SCHULGIN), or bleu lumiere, which has 

 hardly any effect on nuclei. 



The solutions of both these colours should be extremely 

 dilute. They may be used for staining in the mass. 



244. Carmine and Malachite Green. MAAS (Zeit. f. wiss. Zool., x, 

 4, 1890, p. 527 ; Zeit.f. wiss. Mile., viii, 2, 1891, p. 205) recommends borax- 

 carmine followed by weak alcoholic solution of malachite green, with a final 

 washing out with stronger alcohol. 



245. Carmine and Methyl Green (MAX FLESCH, Zool. Anz., 123, 

 1882, p. 554). Sections of cartilage, skin, and glands, made from tissues 

 hardened in Miiller's solution and alcohol, were stained with picro-carmine, 

 and subsequently (not " previously," as erroneously stated in Journ. Roy. 

 Mic. Soc. [N.S.], ii, 1882, p. 883) with an aqueous solution of commercial 

 methyl green made of such a strength that the sections are just distinguish- 

 able in a watch-glassful of the solution when placed on a light ground. 



The method is easy, gives good differentiations, but the stain does not 

 appear likely to be permanent. Mount in balsam. 



246. Picro-carmine and Iodine Green (STIRLING, Journ. Anat. and 

 Physiol, xv, 1881, p. 349, et seq.). 



Iodine green has a specific action on adenoid tissue and mucous glands, 

 which it stains of a bright green. But as methyl green has a similar action, 

 the method appears to be in general superfluous. See " Mucus Glands," in 

 Part II. 



247. Picro-carmine, Kosein, and Anilin Blue; or Picro-car- 

 mine, Anilin Violet, and Anilin Blue ; or Picro-carmine, Anilin 

 Violet, and Iodine Green ; or Picro-carmine, Rosein, and Iodine 

 Green (HENEAGE GIBBES, Journ. Boy. Mic. Soc., iii, 1880, p. 392). H. 

 Gibbes says of these methods that their great utility consists in their 

 power of differentiating glandular structures according to their secretions. 

 In a section of a dog's tongue " the ordinary mucous glands will be found 

 to have taken on a purple colour, while the serous glands which supply the 

 secretion to the taste-organs stain a totally different colour." (See also 

 "Mucus Glands," in Part II.) Most of the differentiations aimed at in the last 

 six sections will probably be better obtained by means of the EHELICH- 

 BIONDI stain given below, 259. 



248. Carmine and Picro-nigrosin (PIANESE), (see Journ. Roy. Mic. 

 Soc., 1892, p. 292). 



