OTHER COMBINATIONS. 165 



aqueous solution of anilin green. Wash with water, wash out with alcohol, 

 and clear with clove oil. 



This combination possesses a rich selectivity, especially for connective and 

 glandular tissues. 



LIST (1. c., 262) recommends' this combination, which he employs in the 

 following manner : Stain for a quarter of an hour in the eosin solution 

 there quoted, rinse with alcohol, and stain for a quarter of an hour in 0'5 

 per cent, aqueous solution of anilin green diluted with three volumes of 

 absolute alcohol. Wash out with absolute alcohol and (as soon as the colour 

 of the eosin begins to reappear) clear with bergamot oil or xylol. 



266. Dahlia and Eosin (SCHIEFFEBDECKEE, 1. c.). Proceed as for 

 anilin green and eosin ( 265), using a 1 per cent, aqueous solution of 

 dahlia. 



267. Methyl Violet and Eosin (SCHIEFFERDECKEK, 1. c.). Proceed as 

 before, using a 1 per cent, aqueous solution of methyl violet. 



268. Baumgarten's Fuchsin and Methylen Blue (Zeit. f. wiss. Mik., 

 i, 1884, p. 415). Stain sections (of chromic objects) for twenty -four hours 

 in a stain made by adding 8 to 10 drops of concentrated alcoholic solution 

 of fuchsin to a watch-glassful of water. Einse with alcohol, and stain for 

 four or five minutes in concentrated aqueous solution of methylen blue, wash 

 out with alcohol for five to ten minutes, and clear with clove oil. Nuclei 

 red, tissues blue, the fuchsin having been driven out of the tissues by the 

 methylen blue, a result which is not attained by washing with alcohol alone, 

 either pure or acidified. 



269. Safranin and Indigo-Carmine (KossiNSKi, Zeit. f. wiss. Mik.> 

 vi, 1, 1880, p. 61). Stain sections ten to twenty minutes in saturated 

 aqueous solution of indigo-carmine, wash with water and with alcohol, and 

 stain with safranin (0'5 per cent, in dilute alcohol), dehydrate and mount. 



Safranin and Nigrosin is a combination also recommended by the same 

 author. Stain for three to five minutes in O'l per cent, aqueous solution of 

 nigrosin, and proceed as before. 



Other Combinations. 



270. Baumgarten's Triple Stain (Bull. Soc. Belg. Mic., 7, 1887, and 

 14, 1888, p. 146 ; Journ. de Microgr., 1888, p. 415 ; Journ. Roy. Mic. Soc., 

 1887, p. 676, and 1889, p. 149). Garbini's Safranin and Anilin Blue 

 (Zool Anz., 1886, p. 26 ; and Zeit.f. wiss. Mik., v, 2, 1888, p. 170). (Both 

 these are much too complicated to be generally recommendable.) Iodine 

 Green and Eosin (STIRLING, Journ. Anat. and Physiol., xv, 1881, p. 354). 

 Rose Bengale and Iodine Green (GRIESBACH, Zool. Anzeig., 135, 1883, 

 p. 172). Rosein and Anilin Blue, Rosein and Anilin Green, Anilin 

 Violet and Anilin Blue, Anilin Violet and Anilin Green (see H. 

 GIBBES, Journ. Roy. Mic. Soc., 1880, p. 391). Metallic Stains and 

 Anilin Colours. Most of the 'coal-tar colours above quoted may be 

 employed after a metallic impregnation. The combination of safranin with 

 gold chloride, first recommended by Pfitzner, is a classical stain. Eosin may 

 usefully be made to follow nitrate of silver impregnation. And other com- 

 binations may be found useful on occasion. 



