CELL-GRANULES. 337 



alcohol (ante, 637), and bringing the ova into one-third 

 glycerin in which is dissolved a little malachite green. The 

 " spheres attractives " stain green. See also BOVERI'S Zellen- 

 Studien (in Jen. Zeitschr. f. Naturw., xxi, 1887, p. 423, or 

 sold separately). 



The osmium and pyroligneous acid method of HEBMANN has been de- 

 scribed in 233. It is said to give a so abundant reduction of the osmium 

 that no other stain is necessary. In the same place HERMANN also recom- 

 mends a modification of the hsematoxylin impregnation method of PAL 

 ( 692) : Testes of Proteus put for twelve to eighteen hours into hsema- 

 toxylin, 1 grm. ; absolute alcohol, 70 c.c. ; water, 30 c.c., in the dark, and 

 washed out for the same time in 70 per cent, alcohol, also in the dark. Sec- 

 tions having been made are treated with dilute pale rose-coloured solution 

 of permanganate of potash till they turn ochre-coloured, rinsed in water, 

 and washed out for removal of the brown peroxide of manganese in Pal's 

 oxalic acid mixture diluted with 5 to 10 volumes of water. They are finally 

 stained for three to five minutes in safranin. 



The iron-haematoxylin stain of BUTSCHLI and that of M. 

 HEIDBNHAIN have been given in 196, 197. 



For BENDA'S copper-hsematoxylin, see Arch. f. mik. Anat., xxx, p. 49 ; 

 also Journ. Boy. Mic. 8oc. ; 1888, p. 158, or Zeit. /. wiss. Mik., v, 1888, 

 p. 499. 



FLEMMING'S orange method has been given 258. 



HENNEGUY'S permanganate method has been given 106. 



RABL'S method (platinum chloride, hasmatoxylin, safranin). 

 See Morph. Jahrb., x, 1884, p. 215. 



PLATNER'S. See Arch. f. mik. Anat., xxxiii, 1889, p. 125. 

 His Kernschwarz has been discussed in 208. 



640. Nucleus of BALBIANI (" Noyau Vitellin," " Cellule Embryo- 

 gene") (Zool. Anz., 1883, p. 659). This may be observed in the fresh state, 

 without the addition of any reagent, in the ova of some animals, amongst 

 others a great number of Arachnida and Myriapoda. It may be brought out 

 more distinctly by treating the ova with a mixture of equal parts of acetic 

 acid and 1 per cent, osmic acid, to which is added a little sodium chloride. 

 This mixture does not render ova so granular as pure dilute acetic acid. 



641. Cell-granules. These for the most part undoubtedly 

 metabolic products are best studied in gland-cells and blood- 

 and lymph-corpuscles, and in certain elements belonging to 

 the group of connective tissues, and the reader is therefore 

 referred to Chap. XXXI for the appropriate methods. 



For ALTMANN'S " Bioblasts/' see, besides that author's 



22 



