CORPUSCLES OF HERBST AND GRANDRY. 345 



The methylen blue impregnation method, 113, et seqq., 

 should also be employed in the study of these nerve-endings. 



650. Innervation of the Muzzle of the Ox (CYBULSKY, Zeit.f. 

 wiss. ZooL, 1883, p. 635; cf. Journ. Roy. Mic. Soc., 1885, p. 

 555). Cybulsky employs the method of HENOCQUE given 

 above, 228. 



651. Tactile Corpuscles (FISCHER, Arch.f. mik. Anat., 1875, 

 p. 366). Fischer employed the gold method of Lowit see 

 224. Ranvier (Traite, p. 918) also recommends this method, 

 as well as his two gold methods, 225, 226. Pieces of skin 

 are first impregnated whole, then hardened by alcohol and 

 sectioned. He finds (as do other authors) that osmic acid 

 and picro-carmine are invaluable aids to the study of these 

 structures, and to that of the corpuscles of Pacini. Purpurin 

 and haematoxylin may also be used for after-staining. See 

 RANVIER, Traite, p. 919; and see also LANGERHANS, Arch, 

 f. mik. Anat., 1873, p. 730; and KULTSCHIZKY, ibid., 1884, 

 p. 358. 



652. Corpuscles of Herbst and Corpuscles of Granary ( CARRIERS, 

 Arch. f. mik. Anat., 1882, p. 146) .Take fresh beaks of ducks, 

 remove the skin and papillas from the margins, and put pieces 

 into formic acid (50 per cent.) for twenty minutes or until 

 transparency is attained ; remove the corneous layer of epi- 

 thelium; rinse in water; gold chloride 1 per cent, (twenty 

 minutes) ; rinse in water ; Pritchard's solution (amyl-alcohol 

 1 per cent., formic acid 1 per cent., water 98 per cent.) from 

 midday till next morning (in the dark) ; rinse in water ; treat 

 with alcohol, imbed in paraffin, and make sections. 



It is important to take only small quantities of gold 

 chloride, not more than about 10 c.c. of the solution to 

 " quite a number" of pieces of skin and papillae. On the 

 other hand, large quantities of Pritchard's solution should be 

 employed. 



DOGIEL (Arch. f. Anat. u. EntwickeL, 1891, p. 182; Zeit.f. 

 wiss. Mik., viii, 4, 1892, p. 520) prefers the methylen blue 

 method (Chap. IX). Four per cent, solution of methylen 

 blue, warmed to 40 C., is injected into blood-vessels of the 

 heads of ducks or geese ; pieces of skin are removed from the 

 beaks, sectioned in pith, and the sections brought on to slides 



