350 MUSCLE AND TENDON. 



CHAPTER XXVIII. 



MUSCLE AND TENDON (NERVE-ENDINGS). 

 Striated Muscle. 



661. Sections (ROLLETT, Denkschr. math, naturw. Kl. k. Acad. 

 Wiss. Wien, 1885; Zeit.f. wiss. Mik., 1886, p. 92). Besides 

 the usual section methods, the following methods of Rollett 

 should be noted: (1) The method mentioned above, 321, 

 of freezing living tissue in white of egg. (2) The same 

 method applied to recently fixed muscle. (3) For hardened 

 muscle, Rollett prefers celloidin. He stains for several hours 

 in Renaut's haematoxylic glycerin diluted with water to a 

 very light violet tint. Dehydrate with alcohol, clear with 

 origanum oil, and mount in dammar. 



662. Dissociation. See Chap. XXIII. 



LANGERHANS' methods for Amphioxus (Arch.f. mik. Anat., 1875, p. 291). 

 For isolation of the muscle-plates macerate the fresh animal in 20 per 

 cent, nitric acid. 



For isolation of the nervous system macerate an animal for three days in 

 20 per cent, nitric acid, then place it for twenty-four hours in water, and 

 shake forcibly. The whole of the nervous system may thus be separated, 

 almost down to the finest peripheral terminations of nerves. 



663. Muscle-cells. For the study of these and allied sub- 

 jects see, inter alia, BEHEENS, KOSSEL, und SCHIEFFERDECKER, 

 Das Mikroskop, &c., vol. ii, pp. 154161 ; also, for the ap- 

 plication of the gold method to the study of muscle- cells, 

 SCHAFER, Proc. Roy. Soc.j xlix, 1891, p. 280; or Journ. Roy. 

 Mic. Soc., 1891, p. 683. 



664. Sarcolemma. Besides the places quoted in last section, 

 see SOLGEK, Zeit. f. wiss. Mik., vi, 2, 1889, p. 189 (small 

 pieces of fresh muscle teased and examined in cold saturated 

 solution of carbonate of ammonia). 



