INNER EAR. 3(31 



may be mounted in dammar or (FLEMMING) in glycerin. 

 RAMON Y CAJAL (1. c. last section) mounts in dammar or colo- 

 phonium varnish without a cover-glass. 



685. Dissociation Methods. For maceration preparations 

 you may use weak solutions (0-2 to 0-5 per cent.) of osmic 

 acid for fixation, and then macerate in 0'02 per cent, chromic 

 acid (M. SCHULTZE), or in iodised serum (M. SCHULTZE), or in 

 dilute^ alcohol (LANDOLT), or in Miiller's solution, or (RANVIER, 

 Traite, p. 957) in pure water, for two or three days. THIN 

 (Journ. ofAnat., 1879, p. 139) obtained very good results by 

 fixing for thirty-six to forty-eight hours in one-third alcohol, 

 or in 25 per cent, alcohol, and then staining and teasing. 



SCHIEFFERDECKER (1. c. 682) macerates fresh retina for 

 several days in the methyl mixture, 548. 



KRAUSE (Intern. Monatsch. f. Anat. u. Hist., 1884, p. 225 ; 

 Zeit.f. wiss. Milt., 1885, pp. 140, 396) recommends treatment 

 for several days with 10 per cent, chloral hydrate solution. 

 Barrett finds that this process preserves the rods and cones 

 admirably. 



Inner Ear. 



686. SCHWALBE (Beitr. z. Phys., 1887; Zeit. f. wiss. Mile., 

 iv, 1, 1887, p. 90; Journ. Boy. Mic. Soc., 1887, p. 840). Fix 

 (cochlea of guinea-pig) for eight to ten hours in " Flemming," 

 wash in water, decalcify (twenty-four hours is enough) in 1 

 per cent, hydrochloric acid, wash the acid out, dehydrate, 

 and imbed in paraffin. 



PRENANT (Intern. Monatsschr. f. Anat. u. Physiol., ix, 1, 

 p. 6; Zeit.f. wiss. Mik., ix, 3, 1893, p. 379). For sections, 

 open the cochlea in solution of Flemming or of Hermann, and 

 fix therein for four to five hours. Avoid decalcification as far 

 as possible, as it is inimical to good preservation of elements ; 

 but if necessary, take 1 per cent, palladium chloride. Make 

 paraffin sections and stain with safranin, or with methyl violet 

 B, or with anilin green and orange, or with Renaut's eosin- 

 haematoxylin. 



Isolation preparations of the stria vascularis may be made 

 by putting a cochlea for a day into 1 per cent, solution of 

 osmic acid, then for four to five days into O'l per cent, solution ; 

 the stria may then be got away whole. 



