368 NERVES. 



adding 1 grm. of hsematoxylin dissolved in a little alcohol to 

 100 c.c. of 2 per cent, acetic acid. They are washed out in 

 saturated solution of carbonate of lithium or sodium. By 

 adding to the carbonate of lithium solution 10 per cent, of a 

 1 per cent, solution of red prussiate of potash, and decolouris- 

 ing therein for two or three hours or more, a finer differen- 

 tiation is obtained. After this the sections are well washed 

 in water and mounted in balsam. 



698. Rossi (Zeit. f. wiss. Mik., vi, 2, 1889, p. 182) also recommends a 

 process stated to be simpler than Weigert's. The tissues are hardened in a 

 liquid composed of water, 100 c.c. ; chromic acid, 075 grm. to 1 grm. ; 

 acetate of copper, 5 grms. Human cord requires six to eight days ; the cord 

 of the dog, three to four ; brain of dog, fifteen to eighteen. They are then 

 dehydrated and imbedded in celloidin. Sections are stained in a liquid made 

 by adding 7 to 8 drops of a 5 per cent, solution of hsematoxylin in absolute 

 alcohol to about 30 c.c. of alcohol. After two hours they are brought into a 

 mixture of 8 drops of hydrochloric acid with 100 c.c. of absolute alcohol, 

 and washed therein until the white substance is seen to be differentiated 

 from the grey. They are washed out for twenty minutes or more in water, 

 dehydrated, and mounted. The sections may be double-stained with borax- 

 carmine. 



699. WOLTERS (Zeit. f. wiss. Mik., vii, 4, 1891, p. 466) pro- 

 ceeds as Kultschitzky, except that he stains in a solution con- 

 taining 2 grms. of heematoxylin (dissolved in a little alcohol) 

 to 100 c.c. of 2 per cent, acetic acid, and kept warm by plac- 

 ing it on the top of a stove kept at 45 C. for twenty-four 

 hours, after which time the sections are dipped in solution of 

 Miiller, and differentiated by the method of Pal. This is 

 an intense myelin stain j medullated fibres blue-black ; ground 

 light ; ganglion-cells yellow. 



700. A second method of WOLTERS, which gave good results 

 with the cerebellum and some peripheral nerves, is as follows : 

 Sections from material hardened in Miiller and cut in 

 celloidin are mordanted for twenty-four hours in solution of 

 acetate of aluminium, or, which is better, in a mixture of 



10 per cent, vanadium chloratum . . 2 parts, 

 8 per cent, aluminium aceticum liquidum . 8 

 washed for five or ten minutes in water, stained in the above- 

 mentioned ha3matoxylin, and differentiated in Weigert's de- 

 colourising mixture. A myelin stain in general, with superb 

 differentiation of the protoplasmic processes of the cells of 

 Purkinje. 



