398 CENTRAL NERVOUS SYSTEM. 



the anilin colour known as Noir Colin. He stains for three to 

 four minutes in a Ol per cent, solution. 



JELGERSMA (Zeit.f. wiss. Mik., 1886, p. 39) finds that anilin 

 blue-black gives excellent results, provided that the English 

 preparation of the colour be alone employed. He makes solu- 

 tions of 1 : 100, 1 : 800, and 1 : 2000, of which the first stains 

 sections in a quarter of an hour, the second in five hours, the 

 third in twelve hours. The stain takes effect on ganglion- 

 cells and their processes, and on axis-cylinders, but does not 

 demonstrate neuroglia or connective tissue. 



SCHMAUS (Munch, med. Wochenschr., No. 8, 1891, p. 147 ; 

 Zeit.f. wiss. Mik. y viii, 2, 1891, p. 230) recommends English 

 blue-black in 0*25 per cent, solution in 50 per cent, alcohol, 

 with the addition of a little picric acid ; sections to be stained 

 for an hour. The addition of picric acid has the advantage of 

 leaving celloidin almost colourless, whilst it stains strongly in 

 pure aqueous solutions of blue-black. 



MARTINOTTI (1. c., 1884, p. 478) finds that picro-nigrosin 

 gives very good results, especially for pathological objects. 

 He stains for two or three hours or days in a saturated 

 solution of nigrosin ( 125) in saturated solution of picric 

 acid in alcohol, and washes out in a mixture of 1 part of 

 formic acid with 2 parts of alcohol until the grey matter 

 appears clearly differentiated from the white to the naked 

 eye. 



758. KAISER (Zeit.f. wiss. Mik., vi, 4, 1889, p. 471) advises, for celloidin 

 sections of spinal cord, naphthylamin brown (obtainable from Griibler). 

 Sections are stained for a few hours in a solution containing 1 part of 

 naphthylamin brown, 200 parts of water, and 100 parts of alcohol, washed 

 with alcohol, cleared with origanum oil, and mounted. Chromophilous 

 ganglion-cells, dark brown ; chromophobous cells, light on a dark ground. 

 Black-ground illumination is recommended. The stain has the advantage 

 of not over-staining, and not staining celloidin to an injurious extent. 



759. The EHRLICH-BIONDI mixture of methyl green, orange, 

 and Saurefuchsin, which has been given in the chapter on 

 " Combination Stains," 259, is a good stain for nerve- 

 centres, especially (Dr. LINDSAY- JOHNSON informs me) if there 

 be added to it about one-third of 20 per cent, (saturated) 

 solution of nigrosin. 



760. GOLGI'S Sublimate Method. Last, but not least, as a 

 general stain we have the sublimate method O/GOLGI (Archivio 



