CARTILAGE STAINS. 4-25 



paper, cleared for a long time in bergamot oil or clove oil, and 

 mounted in xylol balsam. 



This is a double stain ; cartilage, orange ; bone, uncoloured 

 (or green in chromic objects) ; marrow, red. 



BAYERI/S method for ossifying cartilage (Arch. f. mile. Anat., 

 1885, p. 35) is as follows : Portions of ossified cartilage are 

 decalcified as directed 564, cut in paraffin, and stained 

 in Merkel's borax-carmine and indigo-carmine mixture, as 

 directed 242, and mounted in balsam. 



MAX FLESCH (Zeit.f. wiss. Mik., 1885, p. 351) particularly 

 recommends this process for the study of the development of 

 dental tissue. 



MOEENER (Skandinavisches Arch, f. PhysioL, i, 1889, p. 216; 

 Zeit.f. wiss. Mik., vi, 4, 1889, p. 508) gives several stains for 

 tracheal cartilage, chiefly as microchemical tests. 1. Stains 

 for the trabeculse ("Balkennetz"). a. Sections (of alcohol 

 material, I gather) put for half an hour into concentrated 

 aqueous solution of Tropseolin 000, No. 2 (Schuchardt's), 

 and washed out in water until only the trabeculae remain 

 coloured. 6. Stain for a few minutes in concentrated aqueous 

 solution of Sulphindigotate of Potash (not soda), and wash out 

 as above. 



2. Stains for " Chondrinballen" (groups of cartilage- cells) . 

 a. Stain for half a minute to two minutes in 0*15 per cent, 

 aqueous solution of methyl violet, rinse in water, and wash 

 out in 10 per cent, acetic acid until the trabeculae are de- 

 coloured. "Chondrinballen" blue on a colourless ground. 

 &. Stain as above, using a 0'15 per cent, solution of anilin red, 

 and wash out as before. 



3. Combined methods, a. Stain as in la, then as in 2 a> 

 dehydrate quickly, and clear in clove oil. b. Stain as in 1 6, 

 then as in 3 a, but using anilin red instead of methyl violet. 



See a critique of these methods by WOLTERS in Arch. f. mik* 

 Anat., xxxvii, 1891, p. 492; Zeit.f. wiss. Milt., viii, 3, 1891, 

 p. 383 ; also, on the whole subject of cartilage, see SCHIEFPER- 

 DECKER'S Gewebelehre, p. 331. 



Blood. 



802. It might be supposed that for the study of blood it 

 would suffice to prick a finger, place a drop of blood on a 

 slide, cover, and examine it. But this is by no means the 



