428 SOME OTHER HISTOLOGICAL METHODS. 



Fresh (unfixed) blood is perhaps best treated as follows 

 (BizzozEEO and TOEEE, Archivio per le Scienze mediche, vol. iv, 

 No. 18, 1880, p. 390) : Dilute a drop of blood with 0'75 per 

 cent, salt solution in which has been dissolved a little methyl 

 violet. This liquid in no wise affects the form of the elements, 

 stains intensely the nucleus of the red corpuscles, and, in the 

 white, stains the nucleus intensely, and the protoplasm less 

 intensely. May be used for the study of bone-marrow and 

 spleen. 



For the staining of the blood-plates of BIZZOZEKO, this 

 observer (Arch. f. path. Anat. u. Phys. ; Zeit. f. iviss. Mik., 

 1884, p. 389) employs a 0*02 per cent, solution of me- 

 thyl violet in salt solution, or a 1 : 3000 solution of gentian 

 violet. 



TOISON (Journ. Sci. med. de Lille, fev., 1885; Zeit. f. wiss. 

 Mik., 1885, p. 398) recommends that blood be mixed with the 

 following fluid : 



Distilled water .... 160 c.c. 



Glycerin (neutral, 30 Baume) . 30 c.c. 



Pure sulphate of sodium . . 8 grammes. 



Pure chloride of sodium . . 1 gramme. 



Methyl violet 5 B . . . 0'25 

 (The methyl violet is to be dissolved in the glycerin with 

 one half of the water added to it ; the two salts are to be 

 dissolved in the other half of the water, and the two solu- 

 tions are to be mixed and filtered.) White blood-corpuscles 

 stain in this medium in five or ten minutes; the maxi- 

 mum of coloration is attained in from twenty to thirty 

 minutes. White blood- corpuscles, violet ; red blood- corpuscles, 

 greenish. 



FEEEIEE'S liquid is said to have a sp. gr. similar to that of 

 liquor sanguinis. Fuchsin, 1 grm. ; water, 150 c.c.; rectified 

 spirit, 50 c.c. ; dissolve, and add glycerin, 200 c.c. (from 

 Squire's Methods and Formulse, p. 39). 



LECLEKQ'S fuchsin followed by malachite green, or Congo followed by 

 gentian and eosin, see Bull. Soc. Beige de Mic., xvi, 1890, p. 61 ; or Journ. 

 Eoy. Mic. Soc., 1890, p. 675. 



DEKHUYSEN'S methylen blue and acid fuchsin mixture, see Verhandl. 

 Anat. Gesellsch., 1892, p. 90 ; or Journ. Boy. Mic. Soc., 1893, p. 116. 



GEIKSBACH, besides the stains mentioned in the last as being 

 capable of being combined with an osmic acid fixing solution, 



