432 SOME OTHER HTSTOLOGICAL METHODS. 



It is theoretically interesting to observe that hyaline carti- 

 lage, the jelly of Wharton, and the Mastzellen of Ehrlich give 

 the same reactions with basic dyes as raucin does, even their 

 metachromatic reactions being identical. 



These conclusions had already been in part formulated by 

 SUSSDOEP (Deutsche Zeit.f. Thiermed., xiv, pp. 345, 359; see 

 Zeit.f. wiss. Mik., vi, 2, 1889, p. 205). 



See also the important series of papers by BIZZOZERO, 

 " Sulle ghiandole tubulari del tubo gastro-enterico" &c., in the 

 Atti R. Accad. di Sci. di Torino, 1889 to 1892; reports in 

 Zeit.f. wiss. Mik., vii, 1, 1890, p. 61 ; and ix, 2, 1892, p. 219. 

 As regards the safranin reaction, it is well to note that it is 

 not obtained with all brands of the dye ; that of Bindschedler 

 and Busch, in Bale, gives it, whilst safranin of Griibler does 

 not. For the distinctive reactions of old and young mucin 

 see the original, or the last- quoted report of the Zeit.f. wiss. 

 Mik. 



808. " Reticulum " of "Mucus Cells. This was first demonstrated 

 by SCHIEFFERDECZER (Arch. f. mik. Anat., 1884, Heft 3) by means of 

 anilin green. This reaction has given rise to a long polemic between 

 SCHIEFFERDECKER and LIST, for which see Zeit. f. wiss. Mile., 1885, pp. 

 51, 222, 223. According to List, the reticula in question stain in an equally 

 specific way in methyl green, in Bismarck brown, in nitrate of rosanilin (of 

 O'OOOl per cent., ten to fifteen minutes ; see op. cit., iii, 3, 1886, p. 393), and 

 in List's double-stains ( 262, 263). Schiefferdecker, on the contrary, 

 maintains that the reticula demonstrated by the methods of List are not 

 identical with those demonstrated by anilin green. 



809. Goblet Cells. So far as these contain rnucin they give 

 the reactions above described (see FLEMMING, Zeit.f. wiss. Mik., 

 1885, p. 519; and PAULSEN, ibid., p. 520). But the- reactions 

 appear to be different for different animals. Thus PANETH 

 (Arch. f. mik. Anat., xxxi, 1888, p. 113, et seq.) found that in 

 the small intestine of the mouse the contents of the goblet 

 cells did not stain with Bohmer's haematoxylin. And the 

 goblet cells of the small intestine of man did not stain with 

 safranin. 



EANVIEE, in a paper too long to be abstracted here (Comptes rend., 1887, 

 3, p. 145 ; see also Zeit.f. wiss. Mik., v, 2, 1888, p. 233), describes a specific 

 reaction of perrathenic acid (RuOJ on goblet cells. By treating the 

 pharyngeal mucosa of the frog first for ten to twelve hours with vapour of 

 osmium, and then for three minutes with vapours of perruthenic acid, the 

 goblet cells are brought out with remarkable distinctness. The contained 



