COCAIN. 1 1 



I have seen large Medusae very completely anaesthetised in 

 the state of extension in an hour or two by this method. 

 ANDEES finds that this plan does not succeed with Actiniae, as 

 with them maceration of the tissues supervenes before anaes- 

 thesia is established. 



13. Ether and Alcohol maybe administered in the same way. 

 ANDEES has obtained good results with Actiniae by the use of 

 a mixture (invented by SALVATOEE LO BIANCO) containing 20 

 parts of glycerin, 40 parts of 70 per cent, alcohol, and 40 parts 

 of sea-water. This mixture should be carefully poured on to 

 the surface of the water containing the animals, and allowed 

 to diffuse quietly through it. Several hours are sometimes 

 necessary for this. 



EISIG employs alcohol in the same way. 



14. Hydrate of Chloral, which was first recommended, I 

 believe, by Foettinger (Arch, de BioL, vi, 1885, p. 115), gives 

 very good results with some subjects. Foettinger operates 

 by dropping crystals of chloral into the water containing the 

 animals. For Alcyonella he takes 25 to 80 centigrammes of 

 chloral for each hundred grammes of water. It takes about 

 three quarters of an hour to render a colony sufficiently in- 

 sensible to allow of fixing. Foettinger has obtained satis- 

 factory results with marine and fresh-water Bryozoa, with 

 Annelida, Mollusca, Nemertians, Actiniae, and with Astera- 

 canthion. He did not succeed with Hydroids. 



I am bound to state that I have never had the slightest 

 success with Nemertians. 



VEEWOEN (Zeit. f. iviss. Zool., xlvi, 9, 1887, p. 99; see also 

 Journ. Roy. Hie. Soc., 1888, p. 148) operates differently for 

 fresh-water Bryozoa. He puts Cristatella for a few minutes 

 into 10 per cent, solution of chloral, in which the animals 

 sooner or later become extended. 



KUKENTHAL (Zeit. f. wiss. Mik., iv, 8, 1887, p. 878; Journ. 

 Roy. Mic. Soc., 1888, p. 509) has obtained good results with 

 some Annelids, by means of a solution of one part of chloral 

 in 1000 parts of sea-water. 



15. Cocain (RICHARD; Zool. Anz., 196, 1885, p. 332) has 

 been found to give good results. Richard puts a colony of 

 Bryozoa into a watch-glass with 5 cc. of water, and adds 



